Translational_Unit

Part:BBa_K4310012:Design

Designed by: Seungchan Oh   Group: iGEM22_UIncheon   (2022-10-11)


enhanced expression of eGFP by heat shock.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2344
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 372
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3275
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 3554
    Illegal SapI site found at 1
    Illegal SapI site found at 531
    Illegal SapI.rc site found at 516
    Illegal SapI.rc site found at 565


Design Notes

To make compatible to our assembly, CAMV 35S and Tnos are amplified by overhang primers.


Source

pCvHSP70A: BBa_K4310005 Kozak sequence: BBa_K4310003 eGFP: BBa_K431006 Tnos: BBa_J428082 from distribution 2022

References